Source of the photo
http://www.scq.ubc.ca/genome-projects-uncovering-the-blueprints-of-biology/
Author of the description
KÖRINFO
1.: We mark the section of the single stranded DNA that we want to be sequenced by primers.
2.: Beside the normally used reaction mixture we add dideoxy nucleotids, these stops the DNA polymerase enzyme. Each different dideoxy nucleotide is labeled by different fluorescent chemicals, so this way we can identify the terminal nucleotid of the DNA sections.
3. Synthesis and denaturation steps following each other.
4. 2D gel electrophoresis.
5. Detection of the base sequence manually.